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[Rat liver S-adenosyl-L-homocysteine hydrolase purification by affinity column chromatography (author's transl)].

作者信息

Chabannes B, Cronenberger L, Pachéco H

出版信息

Experientia. 1979 Aug 15;35(8):1014-6. doi: 10.1007/BF01949913.

DOI:10.1007/BF01949913
PMID:477860
Abstract

S-adenosyl-L-homocysteine hydrolase (EC 3.3.1.1) has been purified 240-fold from rat liver by affinity column chromatography on aminohexyl sepharose bound 6-mercaptopurine 9 D-riboside. The purified enzyme was homogeneous by gel electrophoresis.

摘要

相似文献

1
[Rat liver S-adenosyl-L-homocysteine hydrolase purification by affinity column chromatography (author's transl)].
Experientia. 1979 Aug 15;35(8):1014-6. doi: 10.1007/BF01949913.
2
Affinity-chromatographic purification of S-adenosyl-L-homocysteine hydrolase. Some properties of the enzyme from rat liver.S-腺苷-L-高半胱氨酸水解酶的亲和层析纯化。大鼠肝脏中该酶的一些特性。
Biochem J. 1981 Feb 1;193(2):503-12. doi: 10.1042/bj1930503.
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Purification of bovine liver S-adenosylhomocysteine hydrolase by affinity chromatography on blue dextran-agarose.
Biochim Biophys Acta. 1988 Apr 14;965(1):22-8. doi: 10.1016/0304-4165(88)90146-8.
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Comparison of some physicochemical and kinetic properties of S-adenosylhomocysteine hydrolase from bovine liver, bovine adrenal cortex and mouse liver.牛肝、牛肾上腺皮质和小鼠肝脏中S-腺苷同型半胱氨酸水解酶的一些物理化学和动力学性质比较。
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5
Solubilization and partial purification of S-adenosyl-L-homocysteine binding proteins from the rat brain.
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S-adenosyl-L-homocysteine: simplified enzymatical preparation with high yield.
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S-Adenosyl-L-homocysteine hydrolase: analogues of S-adenosyl-L-homocysteine as potential inhibitors.S-腺苷-L-高半胱氨酸水解酶：作为潜在抑制剂的S-腺苷-L-高半胱氨酸类似物
Mol Pharmacol. 1977 Sep;13(5):939-47.
8
S-adenosylhomocysteine hydrolase from rat liver.大鼠肝脏中的S-腺苷同型半胱氨酸水解酶。
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[New separation method for nucleoside derivatives of adenosine using high performance liquid chromatography. Application to the determination of S-adenosyl-L-homocysteine hydrolase in the liver of the rat].
J Chromatogr. 1979 Mar 11;170(2):430-6. doi: 10.1016/s0021-9673(00)95471-2.
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Inactivation of S-adenosylhomocysteine hydrolase by 9-beta-D-arabinofuranosyladenine in intact cells.在完整细胞中9-β-D-阿拉伯呋喃糖基腺嘌呤对S-腺苷同型半胱氨酸水解酶的失活作用
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引用本文的文献

1
Affinity-chromatographic purification of S-adenosyl-L-homocysteine hydrolase. Some properties of the enzyme from rat liver.S-腺苷-L-高半胱氨酸水解酶的亲和层析纯化。大鼠肝脏中该酶的一些特性。
Biochem J. 1981 Feb 1;193(2):503-12. doi: 10.1042/bj1930503.
2
S-Adenosylhomocysteine hydrolase from human placenta. Affinity purification and characterization.来自人胎盘的S-腺苷同型半胱氨酸水解酶。亲和纯化及特性鉴定。
Biochem J. 1985 Aug 15;230(1):43-52. doi: 10.1042/bj2300043.

本文引用的文献

1
The enzymatic synthesis of S-adenosyl-L-homocysteine from adenosine and homocysteine.由腺苷和同型半胱氨酸通过酶促合成S-腺苷-L-同型半胱氨酸。
J Biol Chem. 1959 Mar;234(3):603-8.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
In vivo and in vitro metabolism of S-adenosyl-L-homocysteine by Saccharomyces cerevisiae.
Arch Biochem Biophys. 1968 Mar 20;124(1):422-30. doi: 10.1016/0003-9861(68)90347-0.
4
Inhibition of transmethylations of biogenic amines by S-adenosylhomocysteine. Enhancement of transmethylation by adenosylhomocysteinase.S-腺苷同型半胱氨酸对生物胺转甲基作用的抑制。腺苷同型半胱氨酸酶对转甲基作用的增强。
J Biol Chem. 1971 May 25;246(10):3175-81.
5
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
J Biol Chem. 1970 Jun;245(12):3059-65.
6
Metabolism of S-adenosylmethionine in germinating pea seeds: turnover and possible relationships between recycling of sulfur and transmethylation reactions.
Arch Biochem Biophys. 1969 Sep;133(2):216-23. doi: 10.1016/0003-9861(69)90448-2.
7
Methionine metabolism in mammals: synthesis of S-adenosylhomocysteine in rat tissues.
Arch Biochem Biophys. 1973 Nov;159(1):160-5. doi: 10.1016/0003-9861(73)90440-2.
8
Partial purification and characterization of S-adenosylhomocysteine hydrolase isolated from Saccharomyces cerevisiae.从酿酒酵母中分离的S-腺苷高半胱氨酸水解酶的部分纯化及特性研究
J Bacteriol. 1972 Oct;112(1):569-75. doi: 10.1128/jb.112.1.569-575.1972.
9
Methods for the analysis and preparation of adenosylmethionine and adenosylhomocysteine.腺苷甲硫氨酸和腺苷高半胱氨酸的分析与制备方法。
Anal Biochem. 1966 May;15(2):323-33. doi: 10.1016/0003-2697(66)90038-8.
10
A simple general method for the preparation of "6-immobilized" analogues of AMP, ATP, NAD and of other adenine-containing compounds for affinity chromatography.
FEBS Lett. 1973 Dec 1;37(2):134-9. doi: 10.1016/0014-5793(73)80442-9.