一种用于测定5-磷酸核糖-1-焦磷酸的分光光度法。
A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate.
作者信息
Salerno C, Giacomello A, Messina E
出版信息
Experientia. 1979 Aug 15;35(8):1016-7. doi: 10.1007/BF01949914.
PMID:477861
Abstract
A new, sensitive, specific and simple spectrophotometric method for the determination of 5-phosphoribosyl-l-pyrophosphate (PRPP) is presented. PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. At the end of the reaction, PRPP concentration is measured from the extent of conversion of hypoxanthine to inositate. The concentration of the purine base is determined spectrophotometrically in the presence of xanthine oxidase.
摘要
介绍了一种用于测定5-磷酸核糖-1-焦磷酸（PRPP）的新型、灵敏、特异且简便的分光光度法。PRPP在次黄嘌呤-鸟嘌呤磷酸核糖转移酶存在的情况下与过量次黄嘌呤反应。反应结束时，通过次黄嘌呤向次黄苷酸的转化程度来测定PRPP浓度。在黄嘌呤氧化酶存在的情况下，通过分光光度法测定嘌呤碱的浓度。
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