Wallace-Haagens M J, Duffy B J, Holtrop H R
Fertil Steril. 1975 Feb;26(2):175-9.
Sperm survival, in terms of numbers, motility, viability, and metabolic activity, was studied in vaginal washings obtained daily from 22 healthy, fertile, married women during one complete menstrual cycle. The numbers of sperm (counted on Papanicolaou-stained filters) were never large compared to the number of sperm in a single ejaculate. Forty-eight hours after intercourse, only 6% of the specimens showed any evidence of sperm. Motile sperm were observed in only six of 94 postcoital specimens examined within 12 hours after intercourse. Lack of sperm in specimens collected after reported intercourse was associated with contraceptive practices other than the rhythm method, which most of the subjects were using. Fluorochromatic studies of recovered sperm treated with acridine orange indicated sperm viability and phase contrast studies of sperm treated with tetrazolium salts revealed metabolic activity. Our data on sperm motility, numbers, and staining reactions support the conclusion that the small number of sperm that remain in the vagina after intercourse quickly become inactivated.
在一个完整月经周期内，每天从22名健康、可育的已婚女性中获取阴道冲洗液，研究精子在数量、活力、生存能力和代谢活性方面的存活情况。与单次射精中的精子数量相比，阴道冲洗液中的精子数量（在巴氏染色滤片上计数）从来都不多。性交后48小时，只有6%的标本显示有精子存在的迹象。在性交后12小时内检查的94份性交后标本中，仅在6份标本中观察到活动精子。报告性交后收集的标本中缺乏精子与除节律法（大多数受试者使用的方法）以外的避孕措施有关。用吖啶橙处理回收精子的荧光染色研究表明精子具有生存能力，用四氮唑盐处理精子的相差显微镜研究揭示了代谢活性。我们关于精子活力、数量和染色反应的数据支持这样的结论：性交后留在阴道内的少量精子很快就会失活。
