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飞蝗血细胞的组织化学观察

Histochemical observations of the haemocytes of Locusta migratoria.

作者信息

Costin N M

出版信息

Histochem J. 1975 Jan;7(1):21-43. doi: 10.1007/BF01004830.

DOI:10.1007/BF01004830
PMID:47851
Abstract

Five of the six categories of haemocytes of Locusta migratoria, that is, the plasmatocytes, spherule cells, granulocytes, coagulocytes and oenocytoids, contain conspicuous granules of mucosubstance in their cytoplasm. The mucosubstance has been characterized by using a series of histochemical tests, including Alcian Blue staining at different pH levels and salt concentrations, the periodic acid-Schiff (PAS) test, the high iron diamine test, enzymatic digestions and sequential staining methods. The results indicate that four different mucosubstances occur in a granular form, although not all four are found in every blood cell type. The mucosubstances are a neutral glycoprotein and neuraminidase-resistant, sulphated and non-sulphated sialomucins. The non-sulphated sialomucin occurs in both periodate-reactive and -unreactive forms.

摘要

飞蝗血细胞的六个类别中的五个,即浆血细胞、小球细胞、颗粒细胞、凝血细胞和oenocytoids(此词疑有误,可能是oenocytes,暂译为oenocytes样细胞),在其细胞质中含有明显的黏液物质颗粒。通过一系列组织化学测试对黏液物质进行了表征,这些测试包括在不同pH值水平和盐浓度下的阿尔辛蓝染色、过碘酸希夫(PAS)试验、高铁二胺试验、酶消化和连续染色方法。结果表明,有四种不同的黏液物质以颗粒形式存在,尽管并非所有四种都存在于每种血细胞类型中。这些黏液物质是一种中性糖蛋白以及抗神经氨酸酶、硫酸化和非硫酸化的唾液黏蛋白。非硫酸化唾液黏蛋白以高碘酸盐反应性和非反应性两种形式存在。

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Development of primary cell cultures using hemocytes and phagocytic tissue cells of Locusta migratoria: an application for locust immunity studies.利用飞蝗血细胞和吞噬组织细胞建立原代细胞培养物:在飞蝗免疫研究中的应用

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