酿酒酵母中胞嘧啶渗透的特性分析。
Characterization of cytosine permeation in Saccharomyces cerevisiae.
作者信息
Chevallier M R, Jund R, Lacroute F
出版信息
J Bacteriol. 1975 May;122(2):629-41. doi: 10.1128/jb.122.2.629-641.1975.
Abstract
Cytosine permeation in Saccharomyces cerevisiae has been studied. Cytosine uptake is mediated by a permease which is also responsible for purines transport. The Km for the transport of various substrates of this permease have been determined. By means of appropriate selective techniques, mutants with altered Km and mutants lacking the permease have been selected. Cytosine transport is active and is inhibited by 2,4-dinitrophenol, an uncoupler of oxidative phosphorylation, and by N-ethylmaleimide, a reagent of--SH group. Internal labeled cytosine is chased by addition of unlabeled cytosine in the medium. These results support the hypothesis of a carrier-mediated transport, with reduced internal affinity, allowing the release and accumulation of cytosine in the inner compartment. The efflux of cytosine from cytosine permease-less cells has also been studied and shows first order kinetics. A diffusion coefficient of 5.7 per 10- minus 8 cm per S- minus 1 has been evaluated for this efflux.
摘要
已对酿酒酵母中胞嘧啶的渗透进行了研究。胞嘧啶的摄取由一种通透酶介导，该通透酶也负责嘌呤的运输。已确定了这种通透酶对各种底物运输的米氏常数（Km）。通过适当的选择技术，已筛选出Km改变的突变体和缺乏通透酶的突变体。胞嘧啶运输是主动运输，受到氧化磷酸化解偶联剂2,4-二硝基苯酚以及巯基试剂N-乙基马来酰亚胺的抑制。通过在培养基中添加未标记的胞嘧啶来追踪内部标记的胞嘧啶。这些结果支持了载体介导运输的假说，即内部亲和力降低，从而允许胞嘧啶在内部隔室中释放和积累。还研究了无胞嘧啶通透酶细胞中胞嘧啶的流出，其呈现一级动力学。已评估出这种流出的扩散系数为每10的负8次方厘米每秒5.7。
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