Andrews W H, Wagner D, Roetting M J
J Assoc Off Anal Chem. 1979 May;62(3):499-502.
The relative efficiency of trypticase soy broth with added 0.5% K2SO3 and lactose broth as pre-enrichment media for recovering Salmonella from onion powder and garlic powder was collaboratively studied. For each spice, 13 collaborators each received 5 duplicate samples; 4 of the 5 replicate samples were inoculated with 1 of 4 levels of S. thompson ranging, at initiation of analysis, from greater than 3 to 93 organisms/g onion powder and greater than 3 to 43 organisms/g garlic powder. Salmonella growth was inhibited in each of these spices as evidenced by a rapid decline of most probable number values in samples determined immediately after and 7 days following inoculation. Collaborative results of cultural analyses demonstrated superiority of the modified tripticase soy broth for recovering Salmonella in each of the 2 spices. The improved method of detecting Salmonella in onion and garlic powders has been adopted as official first action.
协同研究了添加0.5% K2SO3的胰蛋白胨大豆肉汤和乳糖肉汤作为预增菌培养基从洋葱粉和大蒜粉中回收沙门氏菌的相对效率。对于每种香料,13位协作人员每人收到5个重复样品;5个重复样品中的4个接种了4种水平的汤普森沙门氏菌中的1种,在分析开始时,每克洋葱粉中接种量范围为大于3至93个菌,每克大蒜粉中接种量范围为大于3至43个菌。接种后立即和7天后测定的样品中最可能数的值迅速下降,证明这些香料中的沙门氏菌生长受到抑制。培养分析的协同结果表明,改良的胰蛋白胨大豆肉汤在回收这两种香料中的沙门氏菌方面具有优势。检测洋葱粉和大蒜粉中沙门氏菌的改进方法已被采用为官方首次行动方法。