Perrella M, Samaja M, Rossi-Bernardi L
J Biol Chem. 1979 Sep 25;254(18):8748-50.
The kinetics of formation of the asymmetric carbonmonoxyhemoglobin hybrid (alpha beta)A(alpha beta)C from the parent molecules alpha 2 beta 2A and alpha 2 beta 2C have been studied by electrophoresis at subzero temperatures (down to -40 degrees C) using as supporting media gels of acrylamide/methylacrylate in dimethyl sulfoxide/water mixtures. It has been found that in these media the rate of hybrid formation is markedly affected by pH and decreases by an order of magnitude between pH 7.3 and 8.3. At pH greater than 10, t = -40 degrees C, the hybrid between alpha 2 beta 2A and alpha 2 beta 2C is stable for several hours. A rapid thermal quenching of a mixture of alpha 2 beta 2A and alpha 2 beta 2C prevented hybrid formation during the time required to separate the 2 molecules.
利用丙烯酰胺/甲基丙烯酸酯在二甲亚砜/水混合物中的凝胶作为支持介质，在零下温度（低至-40℃）下通过电泳研究了由母体分子α2β2A和α2β2C形成不对称碳氧血红蛋白杂种（αβ）A（αβ）C的动力学。已发现，在这些介质中，杂种形成速率受pH值显著影响，在pH 7.3至8.3之间下降一个数量级。在pH大于10、t = -40℃时，α2β2A和α2β2C之间的杂种在数小时内稳定。α2β2A和α2β2C混合物的快速热猝灭可在分离这两种分子所需的时间内阻止杂种形成。
