Riccardi C, Puccetti P, Santoni A, Herberman R B
J Natl Cancer Inst. 1979 Oct;63(4):1041-5.
A rapid elimination of tumor cells from some organs was detected in mice following iv injection of tumor cells labeld in vitro with [125I]5-iodo-2'-deoxyuridine. Recovery of radioactivity in different organs (spleen, liver, and lungs) was reduced in mice with high natural killer (NK) cell reactivity in their spleens, as measured in vitro by concomitant short-term 51Cr release assay. Considerable parallelism between in vitro and in vivo reactivities against two mouse lymphomas and a human myeloid cell line was found in mice of different strains and ages. Similarly, various immunophamacologic treatments had comparable effects on in vitro and in vivo reactivities. These findings are consistent with the hypothesis that rapid cytolysis of tumor cells occurred in vivo and that NK cells played a major role in their elimination.
在给小鼠静脉注射经体外[125I]5-碘-2'-脱氧尿苷标记的肿瘤细胞后，检测到某些器官中的肿瘤细胞迅速被清除。通过伴随的短期51Cr释放试验在体外测量发现，脾脏中具有高自然杀伤（NK）细胞反应性的小鼠，不同器官（脾脏、肝脏和肺）中的放射性恢复减少。在不同品系和年龄的小鼠中，发现针对两种小鼠淋巴瘤和一种人髓细胞系的体外和体内反应性之间存在相当大的平行性。同样，各种免疫药理学治疗对体外和体内反应性具有可比的影响。这些发现与以下假设一致：肿瘤细胞在体内发生快速细胞溶解，并且NK细胞在其清除中起主要作用。
