Karanth N G, Bhat S G, Vaidyanathan C S, Vasantharajan V N
Appl Microbiol. 1974 Jan;27(1):43-6. doi: 10.1128/am.27.1.43-46.1974.
The metabolism of the fungicide Dexon (p-dimethylaminobenzenediazo sodium sulfonate) by a soil bacterium is reported for the first time. The organism which is capable of using Dexon only by a co-metabolic process was obtained by enrichment culture and was identified as Pseudomonas fragi. The first metabolic product of Dexon was identified as N,N-dimethyl-p-phenylenediamine. The presence of an enzyme, p-dimethylaminobenzenediazo sodium sulfonate reductase, capable of reducing Dexon to N,N-dimethyl-p-phenylenediamine has been demonstrated in the cell-free extracts of the organism. The enzyme is found to be in the soluble fraction and requires dithiothreitol as a reductant.
首次报道了一种土壤细菌对杀菌剂地可松（对二甲氨基苯重氮磺酸钠）的代谢情况。通过富集培养获得了仅能通过共代谢过程利用地可松的微生物，并鉴定为脆弱假单胞菌。地可松的首个代谢产物被鉴定为N，N - 二甲基对苯二胺。在该微生物的无细胞提取物中已证实存在一种能够将地可松还原为N，N - 二甲基对苯二胺的酶，即对二甲氨基苯重氮磺酸钠还原酶。发现该酶存在于可溶部分，并且需要二硫苏糖醇作为还原剂。
