Sirianni S R, Furukawa M, Huang C C
Mutat Res. 1979 Aug;64(4):259-67. doi: 10.1016/0165-1161(79)90095-5.
A host-mediated assay is described for induction of 8-azaguanine-resistant (azgr) and ouabain-resistant (ouar) mutants in Chinese hamster V79 cells cultured in diffusion chambers (DC) in C3H mice. Injection of the hosts with the indirect mutagen/carcinogen cyclophosphamide (CPP) or 1-(pyridyl-3)-3,3-dimethyltriazene (PyDT) caused a dose-dependent increase in mutation frequency at the loci of azgr and ouar in the V79 target cells. Plating efficiency of V79 cells in DC in mice was decreased depending upon the dose of CPP or PyDT given to the hosts. In addition, the relationship between expression time and mutation frequency was examined and discussed. The data support the use of this system as an effective screening procedure for suspected environmental mutagens or carcinogens, especially those that need to be metabolically activated in vivo.
描述了一种宿主介导的检测方法，用于在C3H小鼠体内扩散小室（DC）中培养的中国仓鼠V79细胞中诱导8-氮杂鸟嘌呤抗性（azgr）和哇巴因抗性（ouar）突变体。给宿主注射间接诱变剂/致癌物环磷酰胺（CPP）或1-（吡啶-3）-3,3-二甲基三氮烯（PyDT）会导致V79靶细胞中azgr和ouar位点的突变频率呈剂量依赖性增加。小鼠体内DC中V79细胞的铺板效率根据给予宿主的CPP或PyDT剂量而降低。此外，还研究和讨论了表达时间与突变频率之间的关系。这些数据支持将该系统用作疑似环境诱变剂或致癌物的有效筛选程序，尤其是那些需要在体内进行代谢活化的物质。
