Yotis W W, Catsimpoolas N, Bergdoll M S, Schantz E J
Infect Immun. 1974 May;9(5):974-6. doi: 10.1128/iai.9.5.974-976.1974.
Purified Staphylococcus aureus enterotoxins B and C(1), the causative agents of staphylococcal food poisoning, were resolved into several components by scanning isoelectric focusing in sucrose density gradients in the absence and presence of 6 M urea and 5 mM dithiothreitol. The observed heterogeneity persisted in the denaturing medium, which suggests that the primary structure of protein enterotoxins B and C(1) may be partly responsible for the isoelectric point differences of the various forms. To our knowledge, the scanning isoelectric focusing patterns of staphylococcal enterotoxins B and C(1) are reported for the first time.
纯化的金黄色葡萄球菌肠毒素B和C（1）是葡萄球菌食物中毒的病原体，通过在不含和含有6M尿素及5mM二硫苏糖醇的蔗糖密度梯度中进行扫描等电聚焦，将其分离为几个组分。在变性介质中观察到的异质性仍然存在，这表明蛋白质肠毒素B和C（1）的一级结构可能部分导致了各种形式的等电点差异。据我们所知，首次报道了葡萄球菌肠毒素B和C（1）的扫描等电聚焦图谱。
