Kozhukharova L
Vet Med Nauki. 1975;12(2):31-8.
Studied was the antigenic relatedness of hyaluronidase contained in the semen of breeder animals of homologic and heterologic species. The experiments were carried out by means of the immunodiffusion and the immunoelectrophoretic methods. The results obtained showed that the seminal hyaluronidase of bulls, rams and bucks is antigenically related, and that of stallions, boars and rabbits does not exhibit antigenic relatedness. Stallion semen is closely related antigenically with the above-mentioned three animal species' semen as manifested by two precipitation bands, but these are not identical with the hyaluronidase precipitation arc. The antigenic relatedness of seminal hyaluronidase is demonstrated by one precipitation line. This fact makes it reasonable to believe that the enzyme activity of hyaluronidase is to be manifested in one protein fraction only, and not in a protein complex. However, further investigations are needed on the biologic activity and the immunologic specificity of hyaluronidase.
研究了同种和异种种用动物精液中所含透明质酸酶的抗原相关性。实验采用免疫扩散法和免疫电泳法进行。所得结果表明，公牛、公羊和公鹿的精液透明质酸酶具有抗原相关性，而种马、公猪和兔子的精液透明质酸酶则未表现出抗原相关性。种马精液与上述三种动物的精液在抗原上密切相关，表现为两条沉淀带，但这些沉淀带与透明质酸酶沉淀弧并不相同。精液透明质酸酶的抗原相关性由一条沉淀线证明。这一事实使人们有理由相信，透明质酸酶的酶活性仅在一种蛋白质组分中表现，而不在蛋白质复合物中表现。然而，关于透明质酸酶的生物活性和免疫特异性还需要进一步研究。
