Perret B A, Seelich T, Furlan M, Beck E A
Thromb Haemost. 1979 Jun 30;41(4):695-701.
In the presence of activated factor XIII, 2-diethylbenzyl-aminoethylthiol-14C-transcinnamate bromide completely inhibited the crosslinking of fibrin. However, all three fibrin chains bound the cinnamic acid, and, in the alpha- and gamma-chains, the binding of label was not restricted to the crosslinking donor sites as might be expected. Furthermore, even in the absence of activated factor XIII, fibrinogen and fibrin incorporated cinnamic acid. Thus, as well as reacting with the functional thiol group of factor XIII, the thiocholine ester of cinnamic acid is incorporated non-specifically throughout the fibrinogen and fibrin subunit chains. The thiocholine ester used differs in this respect from dansyl cadaverine which is incorporated enzymatically and exclusively to the (acceptor) sites involved in crosslinking. Thiocholine ester of cinnamic acid cannot be used as a label for localization of specific crosslinking donor sites.
在活化因子XIII存在的情况下，2-二乙苄基氨基乙基硫醇-14C-反式肉桂酸溴化物完全抑制了纤维蛋白的交联。然而，所有三条纤维蛋白链都结合了肉桂酸，并且在α链和γ链中，标记物的结合并不局限于预期的交联供体部位。此外，即使在没有活化因子XIII的情况下，纤维蛋白原和纤维蛋白也会掺入肉桂酸。因此，除了与因子XIII的功能性硫醇基团反应外，肉桂酸的硫代胆碱酯还会非特异性地掺入整个纤维蛋白原和纤维蛋白亚基链中。在这方面，所使用的硫代胆碱酯与丹磺酰尸胺不同，丹磺酰尸胺是通过酶促方式掺入且仅掺入参与交联的（受体）部位。肉桂酸的硫代胆碱酯不能用作特定交联供体部位定位的标记物。
