用[3-H]脯氨酸对硬骨鱼视觉系统轴突投射进行放射自显影追踪的评估。

Evaluation of [3-H]proline for radioautographic tracing of axonal projections in the teleost visual system.

作者信息

Landreth G E, Neale E A, Neale J H, Duff R S, Braford M R, Northcutt R G, Agranoff B W

出版信息

Brain Res. 1975 Jun 20;91(1):25-42. doi: 10.1016/0006-8993(75)90464-3.

Abstract

The efficacy of [3-H]proline radioautography for tracing retinal ganglion cell projections to the optic tectum of the jewel fish, Hemichromis bimaculatus, has been compared with that of degeneration techniques. There was good agreement between the various methods. Retinal projections to the optic tectum of two other teleosts, the oscar, Astronotus ocellatus, and the goldfish, Carrasius auratus, were examined radioautographically. In addition to conventional methods of analysis, radioautograms were scanned in a slit microdensitometer and by an automated isodensity scanning system. Results of studies with the protein synthesis inhibitor, cycloheximide, are compatible with the suggestion that axonally transported proteins labeled with [3-H]proline may release diffusible precursors that are reincorporated into protein in adjacent regions. The possible advantages and limitations of radioautography of [3-H]proline-labeled axonally transported protein in brief or extended studies are discussed in terms of the results obtained in the teleost visual system.

摘要

已将[3-H]脯氨酸放射自显影术用于追踪宝石鱼（Hemichromis bimaculatus）视网膜神经节细胞向视顶盖投射的功效与变性技术的功效进行了比较。各种方法之间存在良好的一致性。用放射自显影术检查了另外两种硬骨鱼——丽鱼（Astronotus ocellatus）和金鱼（Carrasius auratus）视网膜向视顶盖的投射。除了传统的分析方法外，放射自显影片还在狭缝微密度计和自动等密度扫描系统中进行了扫描。使用蛋白质合成抑制剂环己酰亚胺的研究结果与以下观点相符：即被[3-H]脯氨酸标记的轴突运输蛋白可能释放出可扩散的前体，这些前体又重新掺入相邻区域的蛋白质中。根据硬骨鱼视觉系统中获得的结果，讨论了在短期或长期研究中[3-H]脯氨酸标记的轴突运输蛋白放射自显影术的可能优点和局限性。