Ballou B, Sundharadas G, Bach M L
Science. 1974 Aug 9;185(4150):531-3. doi: 10.1126/science.185.4150.531.
A buffer system conitainin chloral hydrate, taurine, and bromopyridinium lactate was used to dissolve several biological membranes and separate their protein components by polyacrylamide gel electrophoresis. This solvent system was capable of separating molecules of similar size on the hasis of their charge and allows easy recovery of the proteins Thus. aqueous chloral hydrate is an effective solvent for biological membranes.
一种含有水合氯醛、牛磺酸和乳酸溴吡啶鎓的缓冲体系被用于溶解几种生物膜,并通过聚丙烯酰胺凝胶电泳分离其蛋白质成分。这种溶剂体系能够根据电荷分离大小相似的分子,并便于蛋白质的回收。因此,水合氯醛水溶液是一种有效的生物膜溶剂。