Kaighn M E, Babcock M S
Cancer Chemother Rep. 1975 Jan-Feb;59(1):59-63.
The objective of this study was to isolate, characterize, and preserve normal prostatic epithelial and carcinoma cell lines. To date, four specimens have been acquired from patients with benign prostatic hypertrophy. Culture attempts have been successful, yielding a total of 32 cell lines. Three of these lines have been examined thus far and were found to have clearly demonstrable tartrate-inhibitable acid phosphatase granules by histochemical techniques. Several problem areas emerge from these preliminary studies. These involve the difficulty of (a) characterization of the cells as originating from prostatic epithelium, (b) identification of prostatic carcinoma cells, and (c) possible senescence in culture of cell lines.
本研究的目的是分离、鉴定和保存正常前列腺上皮细胞系及癌细胞系。迄今为止，已从良性前列腺增生患者身上获取了4个标本。培养尝试已获成功，共得到32个细胞系。到目前为止，已对其中3个细胞系进行了检测，通过组织化学技术发现它们具有明显可证实的抗酒石酸酸性磷酸酶颗粒。这些初步研究出现了几个问题领域。这些问题包括：(a) 将细胞鉴定为源自前列腺上皮的困难；(b) 前列腺癌细胞的识别；以及(c) 细胞系培养中可能出现的衰老现象。
