金黄色葡萄球菌培养过程中一种复杂形式葡萄球菌激酶的出现。
Occurrence of a complex form of staphylokinase in the course of cultivation of Staphylococcus aureus.
作者信息
Fujimura S, Makino T, Hayashi T T
出版信息
Appl Microbiol. 1974 Jul;28(1):5-10. doi: 10.1128/am.28.1.5-10.1974.
Proteins in the culture supernatant of Staphylococcus aureus PS 47 were subjected to Sephadex chromatography. In the early stage of the cultivation, staphylokinase appeared to have a molecular weight of 15,000 and in the later stage it appeared to have a molecular weight of 320,000. The staphylokinase having a lower molecular weight (type A) converted into one having a higher value (type B) during the course of cultivation. It was demonstrated that conversion of type A into type B took place in vitro (monitored by gel filtration after the two types of staphylokinases were mixed), and it was observed that type B reverted to type A when type B was treated with KCl or detergent. Type B seems to be a complex form of type A and some high-molecular-weight substance.
对金黄色葡萄球菌PS 47培养上清液中的蛋白质进行葡聚糖凝胶色谱分析。在培养早期，葡萄球菌激酶的分子量似乎为15,000，而在后期其分子量似乎为320,000。分子量较低的葡萄球菌激酶（A型）在培养过程中转变为分子量较高的（B型）。已证明A型向B型的转变在体外发生（两种类型的葡萄球菌激酶混合后通过凝胶过滤监测），并且观察到当用KCl或去污剂处理B型时，B型会恢复为A型。B型似乎是A型与某些高分子量物质的复合形式。
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