Karki A B, Kaiser P
Ann Microbiol (Paris). 1979 Feb-Mar;130(2):213-30.
Fours strains of nitrate reducing bacteria isolated from soil were studied for their behavior towards chlorate. They are facultative anaerobes, except for Bacillus megatherium (which is a strict aerobe) and they possess a nitrate reductase A. The growth of three strains of bacteria (Klebsiella pneumoniae, B. licheniformis and Micromonospora globosa) was slowed by sodium chlorate at a concentration of 0.06 to 0.1% while the other strain (B. megatherium) tolerated the CIO3- well. The delay of bacterial growth due to chlorate lasts for a certain period, after which the bacteria multiply again. The lag phase is due to small quantities of chlorite produced from the chlorate; the growth phase which follows is provoked by the multiplication of chlorate resistant mutants, most often nitrate reductase-negative and sometimes positive. Some reverse mutants nitrate reductase positive of K. pneumoniae no longer had the same characteristics as the wild strain: some resisted to chlorate or were different as to gas formation. The reduction of nitrate to ammonia by these bacteria is diminished in the presence of chlorate: the reduction of nitrate to nitrite was inhibited or not inhibited according to the type of strain. The bacteria broke down the chlorate partially or completely, according to the strains and the sustrates.
对从土壤中分离出的四株硝酸盐还原菌进行了氯酸盐行为研究。它们都是兼性厌氧菌，但巨大芽孢杆菌除外（它是严格需氧菌），并且它们都拥有硝酸盐还原酶A。三株细菌（肺炎克雷伯菌、地衣芽孢杆菌和球形小单孢菌）在氯酸钠浓度为0.06%至0.1%时生长减缓，而另一株细菌（巨大芽孢杆菌）对ClO₃⁻耐受性良好。氯酸盐导致的细菌生长延迟持续一定时间，之后细菌再次繁殖。延迟期是由于氯酸盐产生了少量亚氯酸盐；随后的生长期是由耐氯酸盐突变体的繁殖引起的，这些突变体大多是硝酸盐还原酶阴性，有时是阳性。肺炎克雷伯菌的一些硝酸盐还原酶阳性回复突变体不再具有与野生菌株相同的特性：一些对氯酸盐有抗性，或者在产气方面有所不同。在氯酸盐存在的情况下，这些细菌将硝酸盐还原为氨的能力降低：根据菌株类型，硝酸盐还原为亚硝酸盐的过程被抑制或未被抑制。根据菌株和底物的不同，细菌会部分或完全分解氯酸盐。
