Motohara K, Kobayashi M, Ishimoto M
Z Allg Mikrobiol. 1976;16(7):543-50.
Nitrate reductase was investigated in extracts from cells of a chlorate-resistant mutant strain of E. coli which grew anaerobically on nitrate as the sole source of nitrogen. The nitrate reductase was of particulate nature and reduced chlorate like the nitrate reductase from the wild strain, but in contrast was inhibited only weakly by azide or cyanide. Nitrate reductase activity was found in extracts from the mutant cells grown on nitrate as the sole source of nitrogen, but not in extracts from cells grown in complex nutrient medium. Addition of ammonia also caused a decrease in activity. Accordingly, the nitrate reductase in the chlorate-resistant mutant is of the assimilatory type.
对一株大肠杆菌氯酸盐抗性突变菌株细胞提取物中的硝酸还原酶进行了研究，该菌株在以硝酸盐作为唯一氮源的条件下厌氧生长。该硝酸还原酶具有颗粒性质，与野生菌株的硝酸还原酶一样能还原氯酸盐，但与之不同的是，它仅受到叠氮化物或氰化物的微弱抑制。在以硝酸盐作为唯一氮源生长的突变细胞提取物中发现了硝酸还原酶活性，但在复杂营养培养基中生长的细胞提取物中未发现该活性。添加氨也会导致活性降低。因此，该氯酸盐抗性突变体中的硝酸还原酶属于同化型。
