Bhoola K D, Cogdell R
Br J Pharmacol. 1974 Mar;50(3):419-23.
1 The nature of the surface charge on secretory granules from the submaxillary gland of the guinea-pig has been studied by electrophoresis of the isolated granules. The granules suspended in buffered sucrose solutions move to the anode.2 Divalent ions reduce the anodal movements of the granules. Calcium and magnesium neutralize the net surface charge of the granules. This result supports the hypothesis that the electrostatic surface charge which can be reduced by calcium ions may be a major factor in controlling the fusion of secretory granules to the cell membrane, before release by exocytosis.3 The mechanisms which couple membrane events with enzyme or hormone release from secretory glands are considered.
1 通过对分离出的颗粒进行电泳，研究了豚鼠颌下腺分泌颗粒表面电荷的性质。悬浮在缓冲蔗糖溶液中的颗粒向阳极移动。
2 二价离子减少颗粒向阳极的移动。钙和镁中和颗粒的净表面电荷。这一结果支持了以下假说：可被钙离子降低的静电表面电荷可能是在通过胞吐作用释放之前控制分泌颗粒与细胞膜融合的主要因素。
3 考虑了将膜事件与分泌腺中酶或激素释放相联系的机制。
