Wootton T A, Grau C R, Roudybush T E, Hahs M E, Hirsch K V
Arch Environ Contam Toxicol. 1979;8(4):457-63. doi: 10.1007/BF01056352.
Bunker C fuel oil was extracted with petroleum ether and the concentrated extract was given to breeding Japanese quail (Coturnix coturnix Japonica). A dose of extract equivalent to 300 mg bunker C reduced both egg production and hatchability. Fractionation of the extract by absorption on Florisil and elution with petroleum ether followed by chloroform yielded fractions that were tested for toxicity. All the fractions had a pronounced effect upon production; however, only the chloroform eluates were found to affect hatchability of the eggs.
用石油醚萃取船用C级燃料油，将浓缩提取物喂给日本鹌鹑（Coturnix coturnix Japonica）进行繁殖。相当于300毫克船用C级燃料油的提取物剂量降低了产蛋量和孵化率。通过在弗罗里硅土上吸附并先用石油醚洗脱，然后用氯仿洗脱对提取物进行分馏，得到的馏分进行毒性测试。所有馏分对产蛋均有显著影响；然而，仅发现氯仿洗脱液会影响蛋的孵化率。
