细胞表面的周期性变化。II. 细胞松弛素B对同步化中国仓鼠卵巢细胞表面形态的影响。
Cyclic changes in the cell surface. II. The effect of cytochalasin B on the surface morphology of synchronized Chinese hamster ovary cells.
作者信息
Everhart L P, Rubin R W
出版信息
J Cell Biol. 1974 Feb;60(2):442-7. doi: 10.1083/jcb.60.2.442.
Abstract
The surface morphology of Chinese hamster ovary cells treated with cytochalasin B (CB) has been examined using the scanning electron microscope. The cells respond to treatment with CB by retracting peripheral processes, rounding up, and assuming a smooth or gently convoluted surface. This response occurs within minutes. Cells in different stages of the cell cycle all respond in a similar manner. When CB is removed from treated cells by washing with conditioned medium, the cells regain their normal surface conformation within minutes. The surface topography of these released cells is characteristic of their stage in the cell cycle. Because CB causes an alteration in the morphology of the cell surface and because of the speed of the response and recovery, it is proposed that the primary site of action of CB is the cell surface.
摘要
利用扫描电子显微镜对用细胞松弛素B（CB）处理的中国仓鼠卵巢细胞的表面形态进行了检查。细胞对CB处理的反应是缩回周边突起、变圆，并呈现光滑或轻度卷曲的表面。这种反应在数分钟内发生。处于细胞周期不同阶段的细胞均以类似方式做出反应。当用条件培养基洗涤处理过的细胞以去除CB时，细胞在数分钟内恢复其正常的表面形态。这些释放的细胞的表面形貌是其在细胞周期中所处阶段的特征。由于CB会导致细胞表面形态发生改变，且反应和恢复速度较快，因此有人提出CB的主要作用位点是细胞表面。
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引用本文的文献
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2
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In Vitro Cell Dev Biol. 1986 Dec;22(12):725-37. doi: 10.1007/BF02621090.
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本文引用的文献
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Isoleucine-mediated regulation of genome repliction in various mammalian cell lines.异亮氨酸对多种哺乳动物细胞系中基因组复制的调控
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Properties of mitotic cells prepared by mechanically shaking monolayer cultures of Chinese hamster cells.通过机械振荡中国仓鼠细胞单层培养物制备的有丝分裂细胞的特性。
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Cyclic changes in the cell surface. I. Change in thymidine transport and its inhibition by cytochalasin B in Chinese hamster ovary cells.细胞表面的周期性变化。I. 中国仓鼠卵巢细胞中胸苷转运的变化及其被细胞松弛素B的抑制作用
J Cell Biol. 1974 Feb;60(2):434-41. doi: 10.1083/jcb.60.2.434.
8
The effect of cell-to-cell contact on the surface morphology of Chinese hamster ovary cells.细胞间接触对中国仓鼠卵巢细胞表面形态的影响。
J Cell Biol. 1973 Jun;57(3):837-44. doi: 10.1083/jcb.57.3.837.
9
Changes in surface morphology of Chinese hamster ovary cells during the cell cycle.中国仓鼠卵巢细胞在细胞周期中的表面形态变化。
J Cell Biol. 1973 Jun;57(3):815-36. doi: 10.1083/jcb.57.3.815.
10
Effects of deprivation of two essential amino acids on DNA synthesis in Chinese hamster cells.
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