O'Connor T M, Wyttenbach C R
J Cell Biol. 1974 Feb;60(2):448-59. doi: 10.1083/jcb.60.2.448.
The events which occur in the death of visceromotor neurons of the cervical region of the chick embryo's spinal cord have been analyzed by electron microscopy. These normal degenerative events are compared with those in the lumbosacral cord where nerve cell death was induced by removal of peripheral organs. The initial set of degenerative changes include a decrease in nuclear size, the clumping of chromatin beneath the nuclear envelope, an increase in electron opacity of the cells, the disappearance of Golgi bodies, and the disaggregation of polysomes. These events are followed by the loss of the nuclear envelope and most of the endoplasmic reticulum, the appearance of bundles of filaments, and the formation of many ribosome crystals. Ribosome crystals are seen only in the dying cells. Their abundance may indicate a drastic reduction in RNA synthesis as one of the initial events which lead to the death of these neurons. The neurons are finally subdivided and engulfed by cells of the normal glial population, and further breakdown of the cell fragments occurs in large phagocytic vesicles of the gliocytes.
通过电子显微镜分析了鸡胚脊髓颈段内脏运动神经元死亡过程中发生的事件。将这些正常的退行性变化与腰段脊髓中的变化进行了比较，在腰段脊髓中，通过切除外周器官诱导神经细胞死亡。最初的一组退行性变化包括核大小减小、核膜下染色质凝聚、细胞电子密度增加、高尔基体消失以及多核糖体解聚。这些事件之后是核膜和大部分内质网的丧失、细丝束的出现以及许多核糖体晶体的形成。核糖体晶体仅在濒死细胞中可见。它们的大量存在可能表明RNA合成急剧减少，这是导致这些神经元死亡的初始事件之一。这些神经元最终被正常神经胶质细胞群体的细胞细分并吞噬，细胞碎片在神经胶质细胞的大型吞噬泡中进一步分解。
