Merricks D L, Salsbury R L
Appl Microbiol. 1974 Jul;28(1):106-11. doi: 10.1128/am.28.1.106-111.1974.
The dethiomethylation of methionine by a dialyzed extract obtained from the protozoa-rich fraction of rumen fluid is stimulated 2.5-fold by pyridoxal phosphate and strongly inhibited by deoxypyridoxine, a pyridoxal phosphate antagonist. These effects are not seen with undialyzed extracts or with whole rumen fluid. It is suggested that the anaerobic dethiomethylation of methionine by rumen microorganisms requires pyridoxal phosphate as a cofactor.
来自富含原生动物的瘤胃液部分的透析提取物对蛋氨酸的去硫甲基化作用，在磷酸吡哆醛的作用下可被刺激2.5倍，并受到磷酸吡哆醛拮抗剂脱氧吡哆醇的强烈抑制。未透析的提取物或整个瘤胃液未见这些效应。这表明瘤胃微生物对蛋氨酸的厌氧去硫甲基化作用需要磷酸吡哆醛作为辅因子。
