Stanton G J, May D C, de St Jeor S C
Appl Microbiol. 1974 Jul;28(1):145-50. doi: 10.1128/am.28.1.145-150.1974.
Enhanced detection of large-plaque variants of Western equine encephalitis virus in the presence of large numbers of small-plaque Western encephalitis virus was achieved by using higher bicarbonate concentrations in the agar-overlay medium and increasing concentrations of CO(2) in an enclosed environment during the time plaques were developed with neutral red. By this technique we were able to detect large-plaque mutants in cloned small-plaque suspensions for the first time and to show an increase in detectable plaque progeny in plaques developed by neutral red. Further increase in the number of progeny per plaque was determined by assaying extracts of agar overlaying cultures containing known numbers of plaques. The significance of these findings for potential use in mutational studies is discussed.
在存在大量小噬斑西部马脑炎病毒的情况下，通过在琼脂覆盖培养基中使用更高的碳酸氢盐浓度，并在使用中性红形成噬斑的时间段内，在封闭环境中提高二氧化碳浓度，实现了对西部马脑炎病毒大噬斑变体的增强检测。通过这项技术，我们首次能够在克隆的小噬斑悬液中检测到大噬斑突变体，并显示出用中性红形成的噬斑中可检测到的噬斑后代有所增加。通过测定含有已知数量噬斑的琼脂覆盖培养物的提取物，确定了每个噬斑后代数量的进一步增加。讨论了这些发现对于突变研究潜在用途的意义。
