Fedorko M E
J Cell Biol. 1974 Sep;62(3):802-14. doi: 10.1083/jcb.62.3.802.
Mouse peritoneal macrophages in culture for 24 h were exposed to horse [(55)Fe]ferritin and rabbit antihorse [(55)Fe]ferritin antibody complex and the amount of (55)Fe in the medium was assayed up to 2 days after the pulse uptake. Cell survival was assayed by photographing the same areas of the tissue culture Petri dish on successive days and by counting cell numbers per unit area. In experiments in which quantitative assay for cell death is negligible, about 10-20% of the iron ingested by pinocytosis or phagocytosis is released to iron-free medium containing either freshly dialyzed or deironized newborn calf serum (10%). Over the 2-day postpulse period, iron loss is linear. This loss of iron to the medium is significantly reduced by adding iron-saturated newborn calf serum in the postpulse recovery period. A significant portion of the iron released to the medium is bound to transferrin. When human serum is used in the tissue culture system, similar quantities (10-25%) of the ingested iron are lost to the medium 2 days after the pulse.
将培养24小时的小鼠腹腔巨噬细胞暴露于马[（55）铁]铁蛋白和兔抗马[（55）铁]铁蛋白抗体复合物中，并在脉冲摄取后长达2天的时间内测定培养基中（55）铁的含量。通过连续几天拍摄组织培养培养皿的相同区域并计数每单位面积的细胞数量来测定细胞存活率。在细胞死亡定量测定可忽略不计的实验中，通过胞饮作用或吞噬作用摄取的铁中约10%-20%会释放到含有新鲜透析或去铁新生小牛血清（10%）的无铁培养基中。在脉冲后的2天内，铁的损失呈线性。在脉冲后恢复期加入铁饱和的新生小牛血清可显著减少铁向培养基中的损失。释放到培养基中的铁的很大一部分与转铁蛋白结合。当在组织培养系统中使用人血清时，脉冲后2天，摄入的铁也有相似数量（10%-25%）损失到培养基中。
