Thalmann I, Marcus N Y, Thalmann R
Arch Otorhinolaryngol. 1979;224(1-2):89-95. doi: 10.1007/BF00455229.
The levels of the adenine nucleotides ATP, ADP, and AMP in the stria vascularis were measured under normal conditions, and following various durations of ischemia. The concentrations of these compounds were used for the calculation of the adenylate energy charge, the energy status and the phosphorylation state of the stria. Following 10 min of ischemia the adenylate energy charge had decreased three fold, the energy status seven fold and the phosphorylation state 14 fold. To study the potential for recovery of strial function following various brief and prolonged ischemic intervals, a method for the perfusion of the ear via the anterior inferior cerebellar artery was developed. For various reasons it was found advantageous to use "artifical blood" as perfusate, relying upon fluorocarbons as oxygen carriers. The endolymphatic potential was used as electrical indicator of strial function. Recovery of the endolymphatic potential following brief periods of ischemia was paralleled by a corresponding increase of the ATP levels and a drastic decrease of the AMP levels of the stria vascularis. Preliminary results on the effects of substrate-free perfusion are presented.
在正常条件下以及在不同时长的缺血后，测量了血管纹中腺嘌呤核苷酸ATP、ADP和AMP的水平。这些化合物的浓度用于计算腺苷酸能量电荷、血管纹的能量状态和磷酸化状态。缺血10分钟后，腺苷酸能量电荷下降了三倍，能量状态下降了七倍，磷酸化状态下降了十四倍。为了研究在不同短暂和长时间缺血间隔后血管纹功能恢复的潜力，开发了一种通过小脑前下动脉灌注耳朵的方法。由于各种原因，发现使用“人造血液”作为灌注液是有利的，依靠碳氟化合物作为氧载体。内淋巴电位用作血管纹功能的电指标。短暂缺血后内淋巴电位的恢复与血管纹中ATP水平的相应增加和AMP水平的急剧下降同时发生。给出了无底物灌注影响的初步结果。
