Mansour A, Nelson D S
Aust J Exp Biol Med Sci. 1979 Apr;57(2):115-25. doi: 10.1038/icb.1979.12.
The proliferative responses of rat peripheral blood lymphocytes (PBL) and spleen cells to phytohaemagglutinin (PHA) were studied after single or multiple (daily for 4 days) injections of azathioprine (AZ). Lymphopenia developed within 4 h of a single dose (78 mg/kg) of AZ and persisted for at least 72 h. There was no lymphopenia 24 h after the last of 4 daily injections. In vitro, PBL were more sensitive than spleen cells to the inhibitory effect of AZ. Likewise, the responses of PBL were relatively more depressed than those of spleen cells after single or multiple injections of AZ. The degree of depression was less than was expected from the effect of AZ in vitro. Multiple small doses were more depressive than multiple large doses. Serum from treated rats, used at 20% concentration, was more depressive than normal. Thus, rat lymphocytes are quite sensitive to AZ in vitro, but appear to be relatively resistant in vivo, this resistance resembling the resistance of the primary antibody response to AZ treatment.
在单次或多次（连续4天每天一次）注射硫唑嘌呤（AZ）后，研究了大鼠外周血淋巴细胞（PBL）和脾细胞对植物血凝素（PHA）的增殖反应。单次剂量（78 mg/kg）的AZ在4小时内导致淋巴细胞减少，并持续至少72小时。在连续4天每天注射的最后一针后24小时没有淋巴细胞减少。在体外，PBL比脾细胞对AZ的抑制作用更敏感。同样，单次或多次注射AZ后，PBL的反应比脾细胞的反应相对更受抑制。抑制程度低于AZ在体外的作用预期。多次小剂量比多次大剂量更具抑制性。以20%浓度使用的经处理大鼠的血清比正常血清更具抑制性。因此，大鼠淋巴细胞在体外对AZ相当敏感，但在体内似乎具有相对抗性，这种抗性类似于初次抗体反应对AZ治疗的抗性。
