一种分离主要釉原蛋白多肽成分的简单方法。
A simple procedure for the isolation of a major amelogenin polypeptide component.
作者信息
Fincham A G
出版信息
Biochem J. 1979 Jul 1;181(1):171-5. doi: 10.1042/bj1810171.
Abstract
A simple, reproducible gel-filtration procedure for the isolation of one of the major 'J-Group' polypeptides of the bovine foetal dental-enamel matrix is described. The purified polypeptide was characterized by amino acid analysis, electrophoresis, cleavage with CNBr and N-terminal analyses. The isolated component is shown to be closely similar to an amelogenin component described by other workers.
摘要
本文描述了一种简单、可重复的凝胶过滤方法，用于分离牛胎儿牙釉质基质中一种主要的“J组”多肽。通过氨基酸分析、电泳、溴化氰裂解和N端分析对纯化的多肽进行了表征。结果表明，分离出的成分与其他研究人员描述的一种釉原蛋白成分非常相似。
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