Curry J L, Trentin J J
J Exp Med. 1967 Nov 1;126(5):819-32. doi: 10.1084/jem.126.5.819.
The effects of phytohemagglutinin (PHA) were studied in irradiated mice to see if a definite myeloproliferative effect could be demonstrated in vivo. The data obtained suggested the following conclusions. PHA treatment of the bone marrow donor only, causes a consistent but slight reduction in transplantable spleen colony-forming unit (CFU) content of the bone marrow 24 hr after the last PHA injection, but no change was found in the proportion of the various colony types. PHA treatment of the irradiated recipient of normal bone marrow causes no change in the number of spleen colonies. However, 8-day colonies are only about half normal size, are much more likely to be of mixed cell types, contain many large undifferentiated blastoid cells, but fewer transplantable CFU. The spleen sinusoids are packed with hemopoietic cells. Spleen colonies developing in hosts receiving daily injections of PHA show, in addition to the usual spectrum of cell types, a high proportion of unusual blastoid cells resembling the PHA transformed peripheral lymphocytes seen in vitro. The function of these cells is not known, but they may represent augmented proliferation and/or transformation of stem cells. PHA administered after irradiation significantly increased the number of endogenous spleen colonies, and, at certain doses of irradiation, improved postirradiation survival. PHA administered before irradiation had no effect on the number of endogenous spleen colonies formed, or on postirradiation survival. On the basis of these and other data, possible modes of action of PHA are discussed.
研究了植物血凝素（PHA）对受辐照小鼠的影响，以确定是否能在体内证明其明确的骨髓增殖作用。所获得的数据得出了以下结论。仅对骨髓供体进行PHA处理，在最后一次注射PHA后24小时，可使骨髓中可移植的脾集落形成单位（CFU）含量持续但轻微降低，但未发现各种集落类型的比例有变化。对正常骨髓的辐照受体进行PHA处理，脾集落数量无变化。然而，8天的集落大小仅约为正常大小的一半，更有可能是混合细胞类型，含有许多大的未分化胚样细胞，但可移植的CFU较少。脾血窦充满造血细胞。在每天注射PHA的宿主中形成的脾集落，除了通常的细胞类型谱外，还显示出高比例的异常胚样细胞，类似于体外观察到的PHA转化的外周淋巴细胞。这些细胞的功能尚不清楚，但它们可能代表干细胞增殖和/或转化的增强。辐照后给予PHA可显著增加内源性脾集落的数量，并且在一定剂量的辐照下，可改善辐照后的存活率。辐照前给予PHA对形成的内源性脾集落数量或辐照后存活率没有影响。基于这些和其他数据，讨论了PHA可能的作用方式。
