Rule G S, Kruuv J, Lepock J R
Biochim Biophys Acta. 1979 Oct 5;556(3):399-407. doi: 10.1016/0005-2736(79)90128-7.
The initial rate of concanavalin A-mediated agglutination of polyoma transformed Baby Hamster Kidney (pyBHK) cells follows Arrhenius kinetics. There is a smooth decrease in the agglutination rate from 37 degrees C to 22 degrees C with an activation energy of 11.8 +/- 0.2 kcal/mol in this region. There is a sharp decrease in agglutination rate below 22 degrees C. The addition of 0.1 mM 1,3-di-tert-2-hydroxyl-5-methylbenzene, a lipid perturber, increases the agglutination rate by a factor of two and increases the membrane lipid fluidity as determined by the spin label method. The rotational correlation time of the spin label 2N14 (2,2-dimethyl-5-dodecyl-5-methyloxazolidine-N-oxide) was measured. The sum of the enthalpy of activation of rotational diffusion and the enthalpy of activation of translational diffusion is very nearly equal to the enthalpy of activation of agglutination. This is consistent with the rate limiting step of agglutination being receptor diffusion, which is probably limited in pyBHK cells by membrane lipid fluidity.
刀豆球蛋白A介导的多瘤转化幼仓鼠肾（pyBHK）细胞凝集的初始速率遵循阿累尼乌斯动力学。在该区域，凝集速率从37℃平滑下降至22℃，活化能为11.8±0.2千卡/摩尔。在22℃以下，凝集速率急剧下降。添加0.1 mM 1,3-二叔丁基-2-羟基-5-甲基苯（一种脂质扰动剂）可使凝集速率提高两倍，并增加通过自旋标记法测定的膜脂流动性。测量了自旋标记物2N14（2,2-二甲基-5-十二烷基-5-甲基恶唑烷-N-氧化物）的旋转相关时间。旋转扩散活化焓和平动扩散活化焓之和非常接近凝集活化焓。这与凝集的限速步骤是受体扩散一致，在pyBHK细胞中，受体扩散可能受膜脂流动性限制。
