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4-Hydroxy-3-nitrophenylglyoxal. A chromophoric reagent for arginyl residues in proteins.

作者信息

Borders C L, Pearson L J, McLaughlin A E, Gustafson M E, Vasiloff J, An F Y, Morgan D J

出版信息

Biochim Biophys Acta. 1979 Jun 6;568(2):491-5. doi: 10.1016/0005-2744(79)90319-x.

Abstract

The chromophoric reagent, 4-hydroxy-3-nitrophenylglyoxal, is highly selective for the modification of arginine in aqueous solution at pH 7--9. The reagent also inactivates creatine kinase (ATP:creatine N-phosphotransferase, EC 2.7.3.2) in a manner analogous to that reported with phenylglyoxal.

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