Rosenthal M D, Somers K D
Biochim Biophys Acta. 1979 Aug 30;574(2):356-60. doi: 10.1016/0005-2760(79)90017-1.
Phospholipid acyl group retention of normal and mouse sarcoma virus transformed cell lines has been examined. The cells were fed [1-14C]oleate for 24 h and then grown to non-radioactive medium for 4 days. Both the transformed rat and mouse cell lines continued to release 14C-labeled fatty acids and phospholipids into the culture medium similarly to the uninfected cells, and showed shifts in 14C between phospholipid subclasses. Thus, the phospholipid acyl group stability reported for L cells is not characteristic of oncogenic cells in culture.
已对正常细胞系和小鼠肉瘤病毒转化的细胞系的磷脂酰基保留情况进行了检测。用[1-¹⁴C]油酸喂养细胞24小时，然后在无放射性的培养基中培养4天。转化的大鼠和小鼠细胞系与未感染细胞一样，继续向培养基中释放¹⁴C标记的脂肪酸和磷脂，并且¹⁴C在磷脂亚类之间发生了转移。因此，L细胞中报道的磷脂酰基稳定性并非培养中的致癌细胞所特有。
