Holmström B
Appl Microbiol. 1968 Jan;16(1):73-7. doi: 10.1128/am.16.1.73-77.1968.
A method for continuous cultivation of a beta-hemolytic streptococcus, strain H 64, is described. The production of cells and streptokinase at various dilution rates, pH, and temperature were studied in a complex medium supplied with excess glucose. At pH 7.0, productivity of cells and streptokinase, as well as the yield constant with respect to glucose, all increased with increasing dilution rate in the range of 0.1 to 0.5 hr(-1). The production of streptokinase was found to be a function of both growth rate and cell concentration. Although higher concentrations of streptokinase were obtained in experiments with batch cultures, the production of streptokinase in continuous cultures was found to be 2.3 times higher. The possible industrial application of a continuous production method is considered.
描述了一种连续培养β-溶血性链球菌H 64菌株的方法。在供应过量葡萄糖的复合培养基中，研究了不同稀释率、pH值和温度下细胞和链激酶的产生情况。在pH 7.0时，细胞和链激酶的生产力以及相对于葡萄糖的产量常数，在0.1至0.5 hr⁻¹的稀释率范围内均随稀释率的增加而增加。发现链激酶的产生是生长速率和细胞浓度两者的函数。尽管在分批培养实验中获得了更高浓度的链激酶，但连续培养中链激酶的产量被发现高2.3倍。考虑了连续生产方法可能的工业应用。
