测定新产生的混合细菌气溶胶活力的直接方法。
Direct method for determining the viability of a freshly generated mixed bacterial aerosol.
作者信息
Green L H, Green G M
出版信息
Appl Microbiol. 1968 Jan;16(1):78-81. doi: 10.1128/am.16.1.78-81.1968.
A direct method was developed to determine the viability of a freshly generated mixed bacterial aerosol. A mixed suspension of (32)P-labeled Staphylococcus aureus and (35)S-labeled Proteus mirabilis was nebulized, and the aerosol was collected and separated according to particle size with an Andersen sampler. Quantitative and qualitative bacteriological and radioisotopic techniques were used to obtain ratios of bacterial to radioactive counts for each organism in samples of the nebulizer suspension and aerosol. Loss of viability was calculated from the change that occurred between the ratio of the nebulizer suspension and the ratio of the aerosol. The viability of S. aureus was unaffected by aerosolization, whereas the viability of P. mirabilis declined by 20 to 60% and was inversely proportional to particle size. The advantages of this method over present indirect methods, as well as potential applications of the method, are discussed.
开发了一种直接方法来测定新产生的混合细菌气溶胶的生存能力。将（32）P标记的金黄色葡萄球菌和（35）S标记的奇异变形杆菌的混合悬浮液雾化，并用安德森采样器收集气溶胶并按粒径分离。使用定量和定性的细菌学及放射性同位素技术，以获得雾化器悬浮液和气溶胶样品中每种生物体的细菌计数与放射性计数的比率。根据雾化器悬浮液比率和气溶胶比率之间发生的变化计算生存能力的损失。金黄色葡萄球菌的生存能力不受雾化影响，而奇异变形杆菌的生存能力下降了20%至60%，且与粒径成反比。讨论了该方法相对于目前间接方法的优点以及该方法的潜在应用。
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