Satouchi K, Saito K
Biomed Mass Spectrom. 1979 Apr;6(4):144-8. doi: 10.1002/bms.1200060403.
The separation and quantification of the monoenoic isomeric oleic (cis-9-octadecenoic) and cis-vaccenic (cis-11-octadecenoic) acids as their pyrrolidine derivatives, was studied with a gas chromatograph mass spectrometer system using mass chromatography. By monitoring the characteristic ions of the pyrrolidine derivatives of oleic (m/z 208 and 222) and cis-vaccenic (m/z 210 and 224) acids, both derivatives were separated. The ratio of m/z 210 to 208 was proportional to the relative amounts of cis-vaccenic to oleic acid. By this method, the content of cis-vaccenic acid in choline phosphoglycerides from rat liver and Yoshida ascites hepatoma (AH 7974) was determined.
研究了以吡咯烷衍生物形式存在的单烯异构体油酸（顺式-9-十八碳烯酸）和顺式- vaccenic酸（顺式-11-十八碳烯酸）的分离与定量，采用气相色谱-质谱联用系统并使用质量色谱法。通过监测油酸（m/z 208和222）和顺式- vaccenic酸（m/z 210和224）的吡咯烷衍生物的特征离子，两种衍生物得以分离。m/z 210与208的比值与顺式- vaccenic酸与油酸的相对含量成正比。通过该方法，测定了大鼠肝脏和吉田腹水肝癌（AH 7974）中胆碱磷酸甘油酯中顺式- vaccenic酸的含量。
