Juillard G J, Boyer P J, Niewisch H, Hom M
Bull Cancer. 1979;66(3):217-28.
The fate and consequences of intralymphatic injections of cells was investigated in dogs. The distribution of intact radiolabeled cells was determined in vivo by whole body gamma scanning. Comparison of distributions resulting from intralymphatic, subcutaneous, intradermal and intravenous routes of administration showed that the distribution and duration of radiolabel in various organs varied with the route of administration. Following intralymphatic injection, radiolabel was concentrated in first echelon lymph nodes draining the site of injection and was retained in these nodes for over 4 weeks. Histologic studies showed intense cortical and paracortical lymphopoiesis to be associated with the retention of intralymphatically injected tumor cells by first echelon lymph nodes. Serial histologic examination of lymph nodes from intralymphatically injected inbred beagles revealed that the consequent lymphopoiesis persisted for 5 weeks. In vitro evaluation of peripheral blood and lymph node lymphocyte cytotoxicity to the injected cells indicated that retention and nodal lymphopoiesis was associated with the development of direct lymphocyte cytotoxicity. The effects of concommitant tumor burden, cytotoxic drugs and ionizing radiation were also investigated and suggest that the therapeutic potential for use of the intralymphatic route has not yet been realized.
研究了犬体内经淋巴管注射细胞的命运和后果。通过全身γ扫描在体内确定完整放射性标记细胞的分布。比较经淋巴管、皮下、皮内和静脉途径给药后的分布情况表明，放射性标记在各个器官中的分布和持续时间随给药途径而异。经淋巴管注射后，放射性标记集中在引流注射部位的一级淋巴结中，并在这些淋巴结中保留超过4周。组织学研究表明，一级淋巴结对经淋巴管注射的肿瘤细胞的保留与强烈的皮质和副皮质淋巴细胞生成有关。对经淋巴管注射的近交系比格犬的淋巴结进行系列组织学检查发现，随后的淋巴细胞生成持续了5周。对注射细胞的外周血和淋巴结淋巴细胞细胞毒性进行的体外评估表明，保留和淋巴结淋巴细胞生成与直接淋巴细胞细胞毒性的发展有关。还研究了伴随肿瘤负荷、细胞毒性药物和电离辐射的影响，结果表明经淋巴管途径的治疗潜力尚未实现。
