de Graaf F K, Tieze G A, Wendelaar Bonga S, Stouthamer A H
J Bacteriol. 1968 Feb;95(2):631-40. doi: 10.1128/jb.95.2.631-640.1968.
Enterobacter cloacae (strain DF13) was found to produce a bacteriocin which could be induced by mitomycin C. In the supernatant fluid of the induced culture phagelike particles were found. The bacteriocin was partially purified from induced cultures by ammonium sulfate precipitation and gel-filtration on Sephadex G-150. Ultraviolet-absorbing material was eluted from the Sephadex column in three fractions. The biological activity was mainly present in the second fraction and is associated with a protein with a molecular weight of about 61,000. The phagelike particles were found in the first fraction and show no biological activity. Upon conjugation of E. cloacae strain DF13 with another strain of the same species and with Escherichia coli K-12S, the ability to produce bacteriocin was transferred. The new bacteriocinogenic strain produced bacteriocin, which could not be distinguished from that produced by E. cloacae strain DF13. Although transfer of the bacteriocinogenic factor often occurred together with transfer of the ability to produce phagelike particles, it was shown that these two factors are two separate genetic entities. In addition to a bacteriocinogenic factor, E. cloacae strain DF13 was found to carry two other transferable plasmids: one determining resistance against streptomycin and sulfanilamide and another determining resistance against penicillin.
阴沟肠杆菌（菌株DF13）被发现能产生一种可由丝裂霉素C诱导的细菌素。在诱导培养物的上清液中发现了噬菌样颗粒。通过硫酸铵沉淀和在Sephadex G - 150上进行凝胶过滤，从诱导培养物中对细菌素进行了部分纯化。从Sephadex柱上洗脱出了具有紫外吸收的物质，分为三个组分。生物活性主要存在于第二个组分中，与一种分子量约为61,000的蛋白质相关。噬菌样颗粒存在于第一个组分中，且无生物活性。当阴沟肠杆菌菌株DF13与同一物种的另一个菌株以及大肠杆菌K - 12S进行接合时，产生细菌素的能力发生了转移。新的产细菌素菌株产生的细菌素与阴沟肠杆菌菌株DF13产生的细菌素无法区分。尽管细菌素产生因子的转移常常与产生噬菌样颗粒能力的转移同时发生，但已表明这两个因子是两个独立的遗传实体。除了细菌素产生因子外，还发现阴沟肠杆菌菌株DF13携带另外两个可转移质粒：一个决定对链霉素和磺胺的抗性，另一个决定对青霉素的抗性。
