The fine structure of the feline superior vestibular nucleus: identification and synaptology of the primary vestibular afferents.

The superior vestibular nucleus of the cat and its primary vestibular efferents were examined by light and electron microscopy. The primary vestibular afferents branch within the nucleus in a sheet-like pattern, in the transverse plane. The dendritic fields of many secondary neurons are shaped like discs and are also oriented in the transverse plane. This relation between the primary afferents and dendritic fields may be relevant to the convergence of primary afferents innervating particular endorgans onto secondary neurons. Synaptic boutons in the SV were divided into 3 putative types on the basis of the size and shape of their synaptic vesicles. The primary afferent bouton was identified by comparing the SV of the two sides after unilateral lesions of the vestibular ganglion. Its boutons contain round vesicles of 40 nm average diameter and are associated with prominent postsynaptic densities; the two other putative bouton types contain smaller, round vesicles, and pleomorphic vesicles. The primary afferent boutons largely contact proximal dendrites, their appendages, and cell somata of the secondary neurons. In animals receiving unilateral lesions of the vestibular ganglion and allowed to survive long enough for the primary afferent boutons to disappear (5--6 days), there occurs in the denervated as compared to normal SV: (1) a decrease in the fraction of the somal surface of the secondary neurons covered by boutons with small round vesicles; and (2) a decrease in the ratio: number of boutons with small round vesicles to number of boutons with pleomorphic vesicles. In addition, there appears on the lesioned side a new group of boutons with pleomorphic vesicles smaller than those in boutons from the control side. These observations suggest plastic changes in response to deafferentation, and may be related to the marked behavioral recovery which occurs within a few days after lesion of the vestibular ganglion.