Messing R B, Dodge C, Waymire J C, Lynch G S, Deadwyler S A
Brain Res Bull. 1979 Sep-Oct;4(5):615-9. doi: 10.1016/0361-9230(79)90102-3.
3H-thymidine uptake into DNA fractions of rat brain regions was measured following in vivo administration of (methyl-3H)-thymidine and morphine. Acute morphine administration (10 mg/kg; 30 min prior to 3H-thymidine) increased incorporation of 3H-thymidine into DNA of rat striatum. This effect was antagonized by naloxone (1 mg/kg). Further, the observed change in incorporation of 3H-thymidine into DNA in striatum could not be accounted for by differences in the local availability of the label in morphinized rats. An autoradiographic study revealed that the 3H-thymidine was localized in nuclei in cells of the sub-ependymal layer lining the lateral ventricles, an area of glial cell proliferation in adult rats. No change in 3H-thymidine incorporation into DNA was observed in any area of the brain in morphine-addicted rats or in rats undergoing naloxone-precipitated withdrawal. The results indicate that opiates may induce permanent anatomical changes in the brain, including alterations of neuroglial interactions.
在体内给予（甲基 - ³H）- 胸腺嘧啶核苷和吗啡后，测定了³H - 胸腺嘧啶核苷进入大鼠脑区DNA组分的摄取情况。急性给予吗啡（10毫克/千克；在³H - 胸腺嘧啶核苷给药前30分钟）增加了³H - 胸腺嘧啶核苷掺入大鼠纹状体DNA中的量。这种作用被纳洛酮（1毫克/千克）拮抗。此外，在给予吗啡的大鼠中，³H - 胸腺嘧啶核苷掺入纹状体DNA的观察到的变化不能用标记物在局部的可利用性差异来解释。一项放射自显影研究表明，³H - 胸腺嘧啶核苷定位于侧脑室衬里的室管膜下层细胞的细胞核中，这是成年大鼠神经胶质细胞增殖的区域。在吗啡成瘾大鼠或经历纳洛酮诱发戒断的大鼠的大脑任何区域，均未观察到³H - 胸腺嘧啶核苷掺入DNA有变化。结果表明，阿片类药物可能会在大脑中诱导永久性的解剖学变化，包括神经胶质相互作用的改变。
