Dickerson R W, Read R B
Appl Microbiol. 1968 Jul;16(7):991-7. doi: 10.1128/am.16.7.991-997.1968.
An instrument was designed for the study of thermal inactivation of microorganisms using heating times of less than 1 sec. The instrument operates on the principle of rapid automatic displacement of the microorganism to and from a saturated steam atmosphere, and the operating temperature range is 50 to 90 C. At a temperature of 70 C, thermometric lag (time required to respond to 63.2% of a step change) of the fluid sample containing microorganisms was 0.12 sec. Heating time required to heat the sample to within 0.1 C of the exposure temperature was less than 1 sec, permitting exposure periods as brief as 1 sec, provided the proper corrections are made for the lethal effect of heating. The instrument is most useful for heat exposure periods of less than 5 min, and, typically, more than 500 samples can be processed for microbial inactivation determinations within an 8-hr period.
设计了一种仪器用于研究加热时间少于1秒时微生物的热失活情况。该仪器基于微生物在饱和蒸汽环境中快速自动进出的原理运行，工作温度范围为50至90摄氏度。在70摄氏度时，含有微生物的流体样品的热滞后（响应阶跃变化的63.2%所需时间）为0.12秒。将样品加热到暴露温度的0.1摄氏度范围内所需的加热时间少于1秒，只要对加热的致死效应进行适当校正，就可实现短至1秒的暴露时间。该仪器对于少于5分钟的热暴露时间最为有用，通常在8小时内可处理500多个样品以进行微生物失活测定。
