通过区带离心法对哺乳动物细胞的细胞核进行分级分离。
The fractionation of nuclei from mammalian cells by zonal centrifugation.
作者信息
Johnston I R, Mathias A P, Pennington F, Ridge D
出版信息
Biochem J. 1968 Aug;109(1):127-35. doi: 10.1042/bj1090127.
Abstract
- Purified liver nuclei from adult rats separate into two main zones when centrifuged in the slow-speed zonal rotor. One zone contains diploid nuclei, the other tetraploid. 2. The effect of age on the pattern of rat liver ploidy was examined. Tetraploid nuclei are virtually absent from young animals. They increase in proportion steadily with age. Partial hepatectomy disturbs the pattern of ploidy. 3. The zonal centrifuge permits the separation of diploid, tetraploid, octaploid and hexadecaploid nuclei from mouse liver. 4. Rat liver nuclei are isopycnic with sucrose solutions of density 1.35 at 5 degrees .
摘要
- 成年大鼠的纯化肝细胞核在低速区带转子中离心时可分为两个主要区域。一个区域含有二倍体细胞核，另一个区域含有四倍体细胞核。2. 研究了年龄对大鼠肝倍性模式的影响。幼龄动物几乎没有四倍体细胞核。它们的比例随年龄稳步增加。部分肝切除会扰乱倍性模式。3. 区带离心机可将小鼠肝脏中的二倍体、四倍体、八倍体和十六倍体细胞核分离。4. 大鼠肝细胞核在5摄氏度下与密度为1.35的蔗糖溶液等密度。
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