通过氨基酸降解对细菌进行表征。
Characterization of bacteria by their degradation of amino acids.
作者信息
Pickett M J, Pedersen M M
出版信息
Appl Microbiol. 1968 Oct;16(10):1591-5. doi: 10.1128/am.16.10.1591-1595.1968.
Abstract
A procedure for detecting the degradation of amino acids by microorganisms is described, and examples of its use in the characterization of bacteria are presented. The procedure involves inoculating a buffered solution of amino acids with a suspension of bacteria, incubating, chromatographing a sample of the suspension, and detecting degradation in terms of absence of ninhydrin-positive spots.
摘要
本文描述了一种检测微生物对氨基酸降解作用的方法，并给出了其在细菌鉴定中的应用实例。该方法包括用细菌悬液接种氨基酸缓冲溶液，进行培养，对悬液样品进行色谱分析，并根据茚三酮阳性斑点的缺失来检测降解情况。
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