时间和生长培养基对脆弱拟杆菌产生短链脂肪酸的影响。
Effects of time and growth media on short-chain fatty acid production by Bacteroides fragilis.
作者信息
Mayhew J W, Onderdonk A B, Gorbach S L
出版信息
Appl Microbiol. 1975 Apr;29(4):472-5. doi: 10.1128/am.29.4.472-475.1975.
Abstract
Gas-liquid chromatography was used to monitor the evolution of short chain fatty acids by Bacteroides fragilis in five media. Acetic and succinic acids, the prominent end products encountered, were readily detected within 24 h. Propionic, isobutyric, isovaleric, and lactic acids were usually recorded in more limited quantities. Maximum rates of bacterial multiplication, glucose catabolism, and end-production coincided with the first 24 h in carbohydrate-supplemented media. Extended incubation (672 h) favored substantial succinate increases in three of five media. These observations suggest that incubation time and composition of the medium are important determinants in short chain fatty acid production by B. fragilis.
摘要
采用气液色谱法监测脆弱拟杆菌在五种培养基中短链脂肪酸的生成情况。乙酸和琥珀酸是主要的终产物，在24小时内即可轻松检测到。丙酸、异丁酸、异戊酸和乳酸的生成量通常较少。在添加碳水化合物的培养基中，细菌增殖、葡萄糖分解代谢和终产物生成的最大速率均出现在最初的24小时内。延长培养时间（672小时）有利于五种培养基中的三种培养基中琥珀酸大量增加。这些观察结果表明，培养时间和培养基成分是脆弱拟杆菌产生短链脂肪酸的重要决定因素。
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