Baccarani M, Ricci P, Santucci M A, Tura S
Exp Hematol. 1979 Jul;7(6):297-304.
Marrow cells from five normal healthy subjects were cultured in methyl cellulose with and without leucocyte conditioned medium. Cultures were examined daily from day 3 to day 14 for the number of granulocytic aggregates and for their cell composition and mitotic activity. Aggregates of different size (small and large clusters, small and large colonies) displayed four distinct and characteristic patterns of growth. Unidentifiable blast cells were very rare throughout culture. The average proportion of recognizable granulocyte precursors (promyelocytes and myelocytes) (PMC + MC) declined during culture from 60% to 34%. There was a negative correlation between the size of the aggregates and their PMC + MC content, but on any day of culture some aggregates consisted mainly or uniquely of PMC + MC, while some others consisted mainly or uniquely of non-proliferating granulocytes. The mitotic index of PMC + MC was much higher than the one previously determined in vivo, and was significantly higher in large colonies than in other aggregates. These results are consistent with the recent demonstration of different CFU-C (sub)populations. It is suggested that differentiation of the aggregates according to their size and kinetics, coupled with cytological examination, can provide a more refined assessment of the assay, which could be useful for further comparison of normal, abnormal, and leukaemic granulocytopoiesis.
从五名正常健康受试者获取的骨髓细胞，分别在添加和不添加白细胞条件培养基的甲基纤维素中进行培养。从第3天到第14天，每天检查培养物中粒细胞聚集体的数量、细胞组成及其有丝分裂活性。不同大小的聚集体（小簇和大簇、小集落和大集落）呈现出四种不同且具有特征性的生长模式。在整个培养过程中，无法识别的原始细胞非常罕见。可识别的粒细胞前体（早幼粒细胞和中幼粒细胞）（PMC + MC）的平均比例在培养过程中从60%降至34%。聚集体的大小与其PMC + MC含量之间呈负相关，但在培养的任何一天，一些聚集体主要或唯一由PMC + MC组成，而另一些则主要或唯一由非增殖性粒细胞组成。PMC + MC的有丝分裂指数远高于先前在体内测定的数值，并且在大集落中显著高于其他聚集体。这些结果与最近对不同CFU-C（亚）群体的证明一致。有人提出，根据聚集体的大小和动力学进行分化，并结合细胞学检查，可以对该检测方法进行更精细的评估，这可能有助于进一步比较正常、异常和白血病粒细胞生成。
