Shimizu N, Katoh Y, Hida T, Satoh K
Exp Brain Res. 1979 Sep;37(1):139-48. doi: 10.1007/BF01474260.
Combination of glyoxylic acid perfusion and postfixation in permanganate was used in an electron microscopic study of the locus coeruleus (LC) of the rat to give good preservation of fine structure and a reproducible demonstration of noradrenaline (NA) storage granules. Medium-sized LC cells (18 x 30 microns) contained a moderate number of small granular vesicles (SGV) and a few large granular vesicles (LGV), mainly near the Golgi apparatus. Dendritic branches were identified by their SGV content up to the tip. Dendrites were occasionally in close contact with each other or with the soma of LC cells, forming dendro-dendritic or dendro-somatic contacts. Numerous axon terminals containing many SGV and some LGV were observed in the neuropil, and they tended to contact dendrites and somata of LC cells or dendrites of unknown origin. These neuronal contacts were devoid of synaptic specializations except for an array of dense perpendicular lines between the juxtaposed membranes. Small oval cells (10 x 15 microns) devoid of SGV occurred frequently in the peripheral part of the nucleus, and they were occasionally in direct contact with LC cells.
在一项对大鼠蓝斑（LC）的电子显微镜研究中，采用了乙醛酸灌注和高锰酸盐后固定相结合的方法，以良好地保存精细结构并可重复地显示去甲肾上腺素（NA）储存颗粒。中等大小的LC细胞（18×30微米）含有适量的小颗粒囊泡（SGV）和一些大颗粒囊泡（LGV），主要靠近高尔基体。树突分支通过其直至尖端的SGV含量得以识别。树突偶尔相互紧密接触或与LC细胞的胞体接触，形成树突 - 树突或树突 - 胞体接触。在神经毡中观察到许多含有许多SGV和一些LGV的轴突终末，它们倾向于与LC细胞的树突和胞体或未知来源的树突接触。除了并列膜之间的一系列密集垂直线外，这些神经元接触缺乏突触特化。在核的周边部分经常出现没有SGV的小椭圆形细胞（10×15微米），它们偶尔与LC细胞直接接触。
