未处理的同基因小鼠脾细胞对艾氏腹水癌的体内活性。

In vivo activity of spleen cells from untreated syngeneic mice against Ehrlich ascites carcinoma.

作者信息

Jakóbisiak M, Kawiak J, Kawalec M, Kańtoch M, Kamiński M, Rozmysłowicz T

出版信息

Folia Biol (Praha). 1979;25(3):169-78.

Abstract

Inbred CFW/L1 or BALB/c male mice were injected intraperitoneally with 1 X 10(5) EAC cells. They died in 98.6 or 100%, respectively, within 100 days of observation. Intraperitoneal injection of 6 X 10(7) syngeneic spleen cells from 6--9-week-old CFW/L1 or BALB/c donors 2.5--4 h after tumour inoculation significantly prolonged MST and 16.7 or 23.5% of mice, respectively, survived for 100 days. Smaller amounts of spleen cells, thymus cells, mixed spleen and thymus cells, or spleen cells from older donors did not show this effect. Homogenized spleen cells were also inactive. Antitumour activity of spleen cells was still evident when they were isolated from irradiated (2 000R) donors or were given 24 h after tumour inoculation. Spleen cells used for in vivo studies showed spontaneous cytotoxic activity in vitro against EAC cells in the 51Cr-release cell-mediated cytotoxicity test. The possible participation of NK cells in the observed in vivo antitumour activity of normal spleen cells is discussed.

摘要

将1×10⁵个艾氏腹水癌细胞（EAC细胞）腹腔注射给近交系CFW/L1或BALB/c雄性小鼠。在100天的观察期内，它们分别有98.6%或100%死亡。在接种肿瘤2.5至4小时后，腹腔注射来自6至9周龄CFW/L1或BALB/c供体的6×10⁷个同基因脾细胞，显著延长了平均生存时间（MST），分别有16.7%或23.5%的小鼠存活了100天。较少量的脾细胞、胸腺细胞、脾和胸腺混合细胞或来自老年供体的脾细胞均未显示出这种效果。匀浆的脾细胞也没有活性。当从接受过2000伦琴（2000R）照射的供体中分离脾细胞，或在接种肿瘤24小时后给予脾细胞时，其抗肿瘤活性仍然明显。用于体内研究的脾细胞在⁵¹铬释放细胞介导的细胞毒性试验中，对EAC细胞显示出体外自发细胞毒性活性。文中讨论了自然杀伤细胞（NK细胞）可能参与正常脾细胞所观察到的体内抗肿瘤活性。