核糖体生物合成：结构蛋白向50S亚基的非随机添加
Ribosome biogenesis: nonrandom addition of structural proteins to 50S subunits.
作者信息
Sells B H, Davis F C
出版信息
Science. 1968 Mar 15;159(3820):1240-2. doi: 10.1126/science.159.3820.1240.
Assemblage of structural proteins into 50S subunits was examined in Escherichia coli recovering fromt chloramphenicol treatment. Cells previously labeled with H3-leucine for three generations were incuibated for 30 minutes with chloramnlphenicol. Proteins synthesized during the initial 5 minuites of recovery from chlorarmphenicol treatmnent were labeled with C(14)-leucine. Marked variation in the ratios of C(14)- to H(3)-leucine in ribosomal protein occurred in cells that had been treated with chloramphenicol; luntreated cells displayed little variation. Thle resuilts sliggest that ribosomal proteins are assenmbled into 50S subunits in a nonrandom manner.
在从氯霉素处理中恢复的大肠杆菌中，研究了结构蛋白组装成50S亚基的情况。先前用H3-亮氨酸标记三代的细胞用氯霉素孵育30分钟。从氯霉素处理恢复的最初5分钟内合成的蛋白质用C(14)-亮氨酸标记。在用氯霉素处理的细胞中，核糖体蛋白中C(14)-亮氨酸与H(3)-亮氨酸的比例出现显著变化；未处理的细胞变化很小。结果表明核糖体蛋白以非随机方式组装成50S亚基。
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