大鼠胰岛分泌颗粒的分离与特性。II. β颗粒的超微结构。
Isolation and properties of secretory granules from rat islets of Langerhans. II. Ultrastructure of the beta granule.
作者信息
Greider M H, Howell S L, Lacy P E
出版信息
J Cell Biol. 1969 Apr;41(1):162-6. doi: 10.1083/jcb.41.1.162.
Abstract
Beta granules isolated from rat islets of Langerhans and subjected only to phosphotungstic acid had, in negatively stained images, a 50-A periodicity. This periodicity was also observed in thin-section profiles of beta granules in intact cells. In shadowed preparations, the granules were spherical in shape and had irregular edges and surface structure. The presence of such a periodicity in the beta granule indicates that its matrix may be composed of a crystalline material.
摘要
从大鼠胰岛分离出的β颗粒，仅用磷钨酸处理后，在负染色图像中呈现出50埃的周期性。在完整细胞中β颗粒的超薄切片轮廓中也观察到了这种周期性。在投影制备物中，颗粒呈球形，边缘和表面结构不规则。β颗粒中这种周期性的存在表明其基质可能由晶体物质组成。
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