大肠杆菌多核苷酸磷酸化酶突变体的分离与定位
Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli.
作者信息
Reiner A M
出版信息
J Bacteriol. 1969 Mar;97(3):1431-6. doi: 10.1128/jb.97.3.1431-1436.1969.
Abstract
Three strains of Escherichia coli with altered polynucleotide phosphorylase, Q7, Q13, and Q27, were isolated by screening clones from heavily mutagenized cultures for low levels of the enzyme. The three mutations were found to cotransduce with argG and asp, and the pnp locus which they define was mapped with respect to these loci. An explanation for the nonreciprocal cotransduction frequencies observed with asp is provided by the demonstration of an unlinked asp-suppressing locus.
摘要
通过从高度诱变的培养物中筛选低水平该酶的克隆，分离出了三株多核苷酸磷酸化酶发生改变的大肠杆菌菌株，即Q7、Q13和Q27。发现这三个突变与argG和asp共转导，并确定了它们所定义的pnp位点相对于这些位点的图谱。通过证明一个不连锁的asp抑制位点，对观察到的与asp的非相互共转导频率做出了解释。
相似文献
1
Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli.大肠杆菌多核苷酸磷酸化酶突变体的分离与定位
J Bacteriol. 1969 Mar;97(3):1431-6. doi: 10.1128/jb.97.3.1431-1436.1969.
2
Characterization of polynucleotide phosphorylase mutants of Escherichia coli.大肠杆菌多核苷酸磷酸化酶突变体的特性分析。
J Bacteriol. 1969 Mar;97(3):1437-43. doi: 10.1128/jb.97.3.1437-1443.1969.
3
pH-sensitive CDP-diglyceride synthetase mutants of Escherichia coli: phenotypic suppression by mutations at a second site.大肠杆菌的pH敏感型CDP-二甘油酯合成酶突变体：第二位点突变的表型抑制作用
J Bacteriol. 1983 Feb;153(2):731-8. doi: 10.1128/jb.153.2.731-738.1983.
4
Isolation and genetic mapping of Escherichia coli aminopeptidase mutants.大肠杆菌氨肽酶突变体的分离与基因定位
Mol Gen Genet. 1976 Oct 18;148(1):43-7. doi: 10.1007/BF00268544.
5
Mapping of the locus for Escherichia coli transfer ribonucleic acid nucleotidyltransferase.大肠杆菌转移核糖核酸核苷酸转移酶基因座的定位
J Bacteriol. 1974 May;118(2):628-32. doi: 10.1128/jb.118.2.628-632.1974.
6
Suppressor of phosphofructokinase mutations of Escherichia coli.大肠杆菌磷酸果糖激酶突变的抑制因子
J Bacteriol. 1972 Oct;112(1):183-7. doi: 10.1128/jb.112.1.183-187.1972.
7
Study of a suppressor of mutT in Escherichia coli.大肠杆菌中mutT抑制因子的研究。
Mol Gen Genet. 1979 Mar 20;171(2):215-8. doi: 10.1007/BF00270007.
8
Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli.在大肠杆菌中产生天冬酰胺营养缺陷型需要两个非连锁基因发生突变。
J Bacteriol. 1980 Apr;142(1):221-8. doi: 10.1128/jb.142.1.221-228.1980.
9
Genetic mapping of mutation in Escherichia coli leading to a temperature-sensitive RNase D.导致温度敏感型核糖核酸酶D的大肠杆菌突变的遗传图谱分析
Mol Gen Genet. 1982;185(1):142-7. doi: 10.1007/BF00333804.
10
PfkB and pfkC loci of Escherichia coli.大肠杆菌的磷酸果糖激酶B（PfkB）和磷酸果糖激酶C（pfkC）基因座
J Bacteriol. 1975 Jun;122(3):1153-61. doi: 10.1128/jb.122.3.1153-1161.1975.
引用本文的文献
1
Cell-Free PURE System: Evolution and Achievements.无细胞PURE系统：发展历程与成就
Biodes Res. 2022 Aug 30;2022:9847014. doi: 10.34133/2022/9847014. eCollection 2022.
2
Thioflavin T as a fluorescence probe for monitoring RNA metabolism at molecular and cellular levels.硫黄素T作为一种用于在分子和细胞水平监测RNA代谢的荧光探针。
Nucleic Acids Res. 2015 Aug 18;43(14):e92. doi: 10.1093/nar/gkv338. Epub 2015 Apr 16.
3
Post-transcriptional control of gene expression: bacterial mRNA degradation.转录后基因表达调控：细菌 mRNA 降解。
World J Microbiol Biotechnol. 1993 Jul;9(4):421-32. doi: 10.1007/BF00328030.
4
Genetic and biochemical assays reveal a key role for replication restart proteins in group II intron retrohoming.遗传学和生物化学分析揭示了复制重启蛋白在II类内含子逆转归巢中的关键作用。
PLoS Genet. 2013 Apr;9(4):e1003469. doi: 10.1371/journal.pgen.1003469. Epub 2013 Apr 25.
5
Escherichia coli cold-shock gene profiles in response to over-expression/deletion of CsdA, RNase R and PNPase and relevance to low-temperature RNA metabolism.大肠杆菌冷休克基因谱对 CsdA、RNase R 和 PNPase 过表达/缺失的响应及其与低温 RNA 代谢的关系。
Genes Cells. 2012 Oct;17(10):850-74. doi: 10.1111/gtc.12002. Epub 2012 Sep 7.
6
RNA decay: a novel therapeutic target in bacteria.RNA 衰变：细菌治疗的新靶点。
Wiley Interdiscip Rev RNA. 2012 May-Jun;3(3):443-54. doi: 10.1002/wrna.1110. Epub 2012 Feb 28.
7
RNA remodeling and gene regulation by cold shock proteins.冷休克蛋白对 RNA 的重塑和基因调控。
RNA Biol. 2010 Nov-Dec;7(6):788-95. doi: 10.4161/rna.7.6.13482. Epub 2010 Nov 1.
8
Escherichia coli RNase R has dual activities, helicase and RNase.大肠杆菌 RNase R 具有解旋酶和 RNase 的双重活性。
J Bacteriol. 2010 Mar;192(5):1344-52. doi: 10.1128/JB.01368-09. Epub 2009 Dec 18.
9
RNase activity of polynucleotide phosphorylase is critical at low temperature in Escherichia coli and is complemented by RNase II.多核苷酸磷酸化酶的核糖核酸酶活性在大肠杆菌低温环境下至关重要，且可被核糖核酸酶II补充。
J Bacteriol. 2008 Sep;190(17):5924-33. doi: 10.1128/JB.00500-08. Epub 2008 Jul 7.
10
Autogenous regulation of Escherichia coli polynucleotide phosphorylase during cold acclimation by transcription termination and antitermination.在冷驯化过程中，大肠杆菌多核苷酸磷酸化酶通过转录终止和抗终止进行自身调节。
Mol Genet Genomics. 2007 Jul;278(1):75-84. doi: 10.1007/s00438-007-0231-3. Epub 2007 Mar 24.
本文引用的文献
1
Hydrolysis of oligoribonucleotides by an enzyme fraction from Escherichia coli.大肠杆菌中一种酶组分对寡核糖核苷酸的水解作用。
Biochem Biophys Res Commun. 1967 Nov 30;29(4):550-5. doi: 10.1016/0006-291x(67)90520-7.
2
Translation of the genetic message: factors involved in the initiation of protein synthesis.遗传信息的翻译：蛋白质合成起始过程中的相关因素。
Proc Natl Acad Sci U S A. 1966 Jul;56(1):290-5. doi: 10.1073/pnas.56.1.290.
3
Synthesis and breakdown of ribonucleic acid in Escherichia coli starving for nitrogen.在缺乏氮源的大肠杆菌中核糖核酸的合成与分解
Biochim Biophys Acta. 1966 Apr 18;119(1):183-91. doi: 10.1016/0005-2787(66)90049-9.
4
Isolation and characterization of ribonuclease I mutants of Escherichia coli.大肠杆菌核糖核酸酶I突变体的分离与鉴定
J Mol Biol. 1966 Mar;16(1):67-84. doi: 10.1016/s0022-2836(66)80263-2.
5
Specific template requirments of RNA replicases.RNA复制酶的特定模板要求。
Proc Natl Acad Sci U S A. 1965 Aug;54(2):579-87. doi: 10.1073/pnas.54.2.579.
6
Measurement of the unstable RNA in exponentially growing cultures of Bacillus subtilis and Escherichia coli.枯草芽孢杆菌和大肠杆菌指数生长培养物中不稳定RNA的测量。
J Mol Biol. 1968 Jan 28;31(2):237-66. doi: 10.1016/0022-2836(68)90442-7.
7
Genetic locus for ribonuclease I in Escherichia coli.大肠杆菌中核糖核酸酶I的基因座
J Bacteriol. 1969 Mar;97(3):1522-3. doi: 10.1128/jb.97.3.1522-1523.1969.
8
Characterization of polynucleotide phosphorylase mutants of Escherichia coli.大肠杆菌多核苷酸磷酸化酶突变体的特性分析。
J Bacteriol. 1969 Mar;97(3):1437-43. doi: 10.1128/jb.97.3.1437-1443.1969.
9
Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome.用亚硝基胍对复制起点进行诱变：大肠杆菌染色体的复制图谱和模式
J Mol Biol. 1968 May 14;33(3):705-19. doi: 10.1016/0022-2836(68)90315-x.
10
Polymerization of nucleoside diphosphate with a manganese-dependent enzyme from Escherichia coli Q13.利用来自大肠杆菌Q13的一种锰依赖性酶进行核苷二磷酸的聚合反应。
Proc Natl Acad Sci U S A. 1967 Dec;58(6):2468-75. doi: 10.1073/pnas.58.6.2468.
Zotero 插件